Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Apexbio Technology LLC Annexin V-HF647/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes 7-AAD specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot penetrate intact cell membranes However in late-apoptotic and dead cells membrane integrity is compromised allowing 7-AAD to enter and stain nuclear DNA Co-staining with Annexin V-HF647 and 7-AAD thus differentiates cells in various apoptotic stages and dead cells
Apexbio Technology LLC Annexin V-HF488/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes 7-AAD can specifically bind to double-stranded DNA resulting in red fluorescence Normally 7-AAD cannot pass through intact cell membranes however it can penetrate the cell membranes of late-stage apoptotic and dead cells staining their nuclei Combined staining with Annexin V-HF488 and 7-AAD allows differentiation of cells in various stages of apoptosis and dead cells
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (HF594) 100tests
EdU Flow Cytometry Assay Kits (HF594) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The EdU Flow Cytometry Assay Kits (HF594) use HyperFluor 594 azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (HyperFluor 594 azide excitation maximum at 590 nm emission maximum at 617 nm)
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
Sigma Aldrich Fine Chemicals Biosciences Iscoves Modified Dulbeccos
ISCOVES Iscoves Modified Dulbeccos
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X without K, pH 7.4) - 1L
Phosphate Buffered Saline PBS (10X without K pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
FUJIFILM Biosciences MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Ethos Biosciences Dilurex 10X PBS Buffer, 100ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661558.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is offered in a large, ready-to-use format with 1.0mL pre-filled tubes, making it suitable for laboratory applications that require rapid, high-volume processing. With 1000 tubes per case, this product is ideal for clinical laboratories, research facilities, and biotechnological applications that perform large-scale sample analysis. PBS helps maintain osmotic pressure and pH stability, which is essential for preserving cell integrity during experiments. The pre-filled tubes offer convenience and reduce contamination risks, making them ideal for use in microbiology, molecular biology, and cell culture studies where consistent buffer conditions are required.
Zymo Research Corporation RBC Lysis Buffer, 50 ml
RBC Lysis Buffer (50 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
Abcam PBS Tablets (1000 ml), 10TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
FUJIFILM Biosciences PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.